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- W2389783921 abstract "A method suitable for extraction of cotton mitochondria and mitochondrial DNA(mtDNA)is reported in details.Mitochondria is separated by centrifugation in a sucrosestep density gradient and purified through DNase I digestion. mtDNA is extracted withchloroform. PVP and DIECA are used to bind phenolic compounds and danin and to inacti-vate phenol oxidases,respectively. Many operation steps are greatly improved in this pro-tocol.mtDNA prepared by this method for restriction analvsis,PCR and other molecularoperations were successfully used." @default.
- W2389783921 created "2016-06-24" @default.
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- W2389783921 date "1996-01-01" @default.
- W2389783921 modified "2023-09-23" @default.
- W2389783921 title "Purification of Cotton Mitochondria and Mitochondrial DNA" @default.
- W2389783921 hasPublicationYear "1996" @default.
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