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- W2389792295 abstract "In this work,the Pichia pastoris expression system was applied to express the T.reesei EGⅣ.The eg4 gene was isolated from rice hull induced T.reesei culture through RT-PCR,and was ligated with the Pichia expression vector pPICZαA,resulting in the recombinant plasmid pPICZαA-eg4.The recombinant plasmid pPICZαA-eg4 was then linearized and transformed into P.pastoris GS115,and the eg4 gene was in frame integrated into the Pichia genome through homologous recombination,resulting the recombinant strain P.pastoris-EGⅣ1.With methanol induction,the recombinant strain P.pastoris-EGⅣ1 expressed and secreated EGⅣ into the culture supernatant with CMC activity of 2.11U/mL.The SDS-PAGE analysis showed that the apparent molecular weight of expressed protein was about 50kD,slightly less than that produced by T.reesei." @default.
- W2389792295 created "2016-06-24" @default.
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- W2389792295 date "2005-01-01" @default.
- W2389792295 modified "2023-09-25" @default.
- W2389792295 title "Expression of the Trichoderma reesei Endoglucanse IV in Pichia pastoris" @default.
- W2389792295 hasPublicationYear "2005" @default.
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