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- W2389794179 abstract "Objective To clone,express and conduct a preliminary immunoreacticity study on genes of Taenia saginata asiatica's actin related protein 2/3 complex subunit 4(Arp2/3).Methods Based on the Arp2/3 sequence template in the library of cDNA of Taenia asiatica,a pair of special primer was synthesized to amplify the gene through PCR technology.The genes were cloned into prokaryotic expression vector Pet-28α(+)inducing the target genes to conduct expression through IPTG in competent Escherichia coli BL21/DE3 processed by CaCl2.The products of the expression was identified with SDS-PAGE.As the expression took place in an inclusion body,SKL was used to achieve purified recombinant protein and the immunoreacticity study was conducted with Western-blotting.Results PCR,double enzyme digestion and DNA sequencing indicated that pET-28a(+)and Arp2/3 recombinant plasmid was successfully constructed.SDS-PAGE results showed that the gene expression took place in Escherichia coli BL21/DE3,and highly pure protein was achieved after the dissolving,refolding and particle exchange chromatography of inclusion body deposits.The recombinant protein reacted with Taenia asiatica and taeniarhynchus saginatus infected patients' serum,which indicated the immunoreacticity of the protein.Conclusion The Arp2/3 subunit 4 gene is successfully cloned.The recombinant protein is obtained through expression and purification,and the gene's immunoreacticity is confirmed,which provides the foundation for further studies of the gene." @default.
- W2389794179 created "2016-06-24" @default.
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- W2389794179 date "2009-01-01" @default.
- W2389794179 modified "2023-09-23" @default.
- W2389794179 title "Cloning and prokaryotic expression of actin related protein 2/3 complex subunit 4 of Taenia saginata asiatica" @default.
- W2389794179 hasPublicationYear "2009" @default.
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