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- W2389797881 abstract "Aim To evaluate the manner and molecular mechanism of HeLa cell death induced by Coxsackie virus B3(CVB3).Methods After co -cultivated with CVB3,HeLa cells were harvested at different time and detected by contrast microscopy,electron microscopy,FACS and molecular biological measures of cy topathic changes,as well as mRNA and protein expression of caspase -3gene.Results Degeneration and necrosis soon arised in HeLa cells,a nd more apoptotic cells occured till24hours after the cells were co -cultivated with CVB3;within 6hours after the co -cultivation,caspase -3mRNA increased rapidly,reaching a peak value,and then fell to approac h the level before the co -culti-vation;caspase -3protein gradually increased within 42hours.Con-clusion CVB3may induce apoptosis and necrosis in HeLa cells,and the latter appears much earlier.Apopto sis of HeLa cells is closely correlat ed with the expression of caspase -3." @default.
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- W2389797881 date "2001-01-01" @default.
- W2389797881 modified "2023-09-26" @default.
- W2389797881 title "Coxsackievirus B3-Induced Apoptosis in HeLa Cells Dependent of Caspase" @default.
- W2389797881 hasPublicationYear "2001" @default.
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