Matches in SemOpenAlex for { <https://semopenalex.org/work/W2389803391> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2389803391 abstract "Objective:To investigate the antigen-specific humoral and cellular immune responses induced by HBV-preS2/S-C3d3 combined DNA vaccines.Methods:Raji cell binding assay was performed to evaluate the expressed protein.Female BALB/c mice were primed by i.m. gene immunization with different recombinant plasmids three times at three-week interval.The levels and avidity of specific IgG in sera collected at the indicated times from each group were determined by ELISA.Splenocytes from immunized mice were stimulated with hCGβ protein and then harvested to analyze specific lympho-proliferative response by ~3H-Thymidine incorporation assay.The mRNA levels of IL-4,IFN-γ,T-bet and GATA-3 were detected by semi-quantitative RT-PCR.Results:preS2/S-C3d3 fusion proteins could bind CR2 positive Raji cells.The levels and avidity of HBsAg specific IgG,lympho-proliferative response in the mice immunized with TR421-preS2/S-C3d3 were significant higher than those in TR421-preS2/S-immunized mice.The increase of IL-4,T-bet and GATA-3 mRNA levels was detected in mice with preS2/S-C3d3-expressing DNA compared with mice with preS2/S-expressing DNA,but the IFN-γ mRNA level decreased.Conclusion:C3d could enhance both humoral and cellular immune responses against HBV following gene immunization by binding CR2,upregulating the expression of IL-4 and accelerating the avidity maturation of anti-HBs IgG." @default.
- W2389803391 created "2016-06-24" @default.
- W2389803391 creator A5017683985 @default.
- W2389803391 date "2006-01-01" @default.
- W2389803391 modified "2023-09-22" @default.
- W2389803391 title "The antigen-specific immune responses induced by HBV-preS2/S-C3d3 combined DNA vaccine" @default.
- W2389803391 hasPublicationYear "2006" @default.
- W2389803391 type Work @default.
- W2389803391 sameAs 2389803391 @default.
- W2389803391 citedByCount "0" @default.
- W2389803391 crossrefType "journal-article" @default.
- W2389803391 hasAuthorship W2389803391A5017683985 @default.
- W2389803391 hasConcept C104317684 @default.
- W2389803391 hasConcept C147483822 @default.
- W2389803391 hasConcept C153911025 @default.
- W2389803391 hasConcept C159047783 @default.
- W2389803391 hasConcept C159654299 @default.
- W2389803391 hasConcept C16232356 @default.
- W2389803391 hasConcept C167508917 @default.
- W2389803391 hasConcept C179223381 @default.
- W2389803391 hasConcept C203014093 @default.
- W2389803391 hasConcept C2522874641 @default.
- W2389803391 hasConcept C2777410769 @default.
- W2389803391 hasConcept C2780593183 @default.
- W2389803391 hasConcept C2780801004 @default.
- W2389803391 hasConcept C2780868878 @default.
- W2389803391 hasConcept C40767141 @default.
- W2389803391 hasConcept C55493867 @default.
- W2389803391 hasConcept C86803240 @default.
- W2389803391 hasConcept C8891405 @default.
- W2389803391 hasConceptScore W2389803391C104317684 @default.
- W2389803391 hasConceptScore W2389803391C147483822 @default.
- W2389803391 hasConceptScore W2389803391C153911025 @default.
- W2389803391 hasConceptScore W2389803391C159047783 @default.
- W2389803391 hasConceptScore W2389803391C159654299 @default.
- W2389803391 hasConceptScore W2389803391C16232356 @default.
- W2389803391 hasConceptScore W2389803391C167508917 @default.
- W2389803391 hasConceptScore W2389803391C179223381 @default.
- W2389803391 hasConceptScore W2389803391C203014093 @default.
- W2389803391 hasConceptScore W2389803391C2522874641 @default.
- W2389803391 hasConceptScore W2389803391C2777410769 @default.
- W2389803391 hasConceptScore W2389803391C2780593183 @default.
- W2389803391 hasConceptScore W2389803391C2780801004 @default.
- W2389803391 hasConceptScore W2389803391C2780868878 @default.
- W2389803391 hasConceptScore W2389803391C40767141 @default.
- W2389803391 hasConceptScore W2389803391C55493867 @default.
- W2389803391 hasConceptScore W2389803391C86803240 @default.
- W2389803391 hasConceptScore W2389803391C8891405 @default.
- W2389803391 hasLocation W23898033911 @default.
- W2389803391 hasOpenAccess W2389803391 @default.
- W2389803391 hasPrimaryLocation W23898033911 @default.
- W2389803391 hasRelatedWork W2253908234 @default.
- W2389803391 hasRelatedWork W2350956107 @default.
- W2389803391 hasRelatedWork W2352550444 @default.
- W2389803391 hasRelatedWork W2353054929 @default.
- W2389803391 hasRelatedWork W2356017175 @default.
- W2389803391 hasRelatedWork W2360379108 @default.
- W2389803391 hasRelatedWork W2361652624 @default.
- W2389803391 hasRelatedWork W2363407359 @default.
- W2389803391 hasRelatedWork W2364694726 @default.
- W2389803391 hasRelatedWork W2375222593 @default.
- W2389803391 hasRelatedWork W2377210555 @default.
- W2389803391 hasRelatedWork W2385343684 @default.
- W2389803391 hasRelatedWork W2389001551 @default.
- W2389803391 hasRelatedWork W2407360137 @default.
- W2389803391 hasRelatedWork W2419319793 @default.
- W2389803391 hasRelatedWork W2419481213 @default.
- W2389803391 hasRelatedWork W3142123149 @default.
- W2389803391 hasRelatedWork W64755026 @default.
- W2389803391 hasRelatedWork W992844922 @default.
- W2389803391 hasRelatedWork W3030542168 @default.
- W2389803391 isParatext "false" @default.
- W2389803391 isRetracted "false" @default.
- W2389803391 magId "2389803391" @default.
- W2389803391 workType "article" @default.