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- W2389831437 abstract "On the basis of the nucleotide sequences of porcine Mx1 gene(poMx1)available in GenBank,a pair of primers was designed and synthesized to amplify a specific 115 bp nucleotide fragment from Mx1 full-length gene.Then,the SYBR Green Ⅰ real-time PCR assay was established based on 10-fold dilution recombinant plasmid as the amplification template after it was cloned and sequenced.The results showed that the standard curve regression equation was Y=-2.986 3 lg X+38.239 3(R2=0.998 5),and that the standard curve was of high linearity,specificity,sensitivity and reproducibility.The production of poMx1 mRNA from virus-infected cells was quantitatively analyzed after swine kidney cells(PK-15)was stimulated by classical swine fever virus(HCLV strain).The data showed the production of poMx1 mRNA was much more after 4 h(P0.01),and then slowly reduced as time grew.So the results showed that this PCR assay could be applied to detect the expression level of poMx1 mRNA." @default.
- W2389831437 created "2016-06-24" @default.
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- W2389831437 date "2013-01-01" @default.
- W2389831437 modified "2023-09-23" @default.
- W2389831437 title "Establishment and application of the SYBR Green I real-time PCR assay for detection of porcine antiviral protein Mx1 gene" @default.
- W2389831437 hasPublicationYear "2013" @default.
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