Matches in SemOpenAlex for { <https://semopenalex.org/work/W2389837344> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2389837344 abstract "Drosophila SCS' insulator was inserted upstream,downstream,or both up-and down-stream of an egfp gene expression cassette driven by CMV promoter,in different directions.They were then inserted into baculovirus genome to construct eight recombinant viruses,plus a control virus.The expression of egfp was compared in mammalian cell lines CHO,BHK and CNE transduced by these viruses.Although these viruses replicated to a similar level in insect Sf9 cells,the level of egfp expression mediated by these viruses varied significantly in virus-transduced mammalian cells.The expression level was highest when SCS' was placed upstream of the expression cassette in the same orientation as egfp gene.In CHO cells,EGFP level expressed by this virus was almost 3 times of the control virus,while the egfp mRNA was also 4 times higher than the control virus.When one SCS' was inserted upstream of the egfp gene in the same direction as the gene,while another SCS' downstream of the egfp gene in the opposite direction,the expression level of EGFP was only 1/3 of the control virus,and the egfp mRNA as 1/6—1/5 of the control virus too.Presence of Sodium butyrate and TSA,as the inhibitors of histone deacetylase in the medium,singnicantly improved the expression of EGFP by this low expression virus,with the expression level surpassed the control virus.These results indicated that the gene expression mediated by baculovirus was affected by the regulating mechanism of cellular chromatin structure,and provided an approach to improve the expression effect of baculovirus as a mammalian cell gene expression vector." @default.
- W2389837344 created "2016-06-24" @default.
- W2389837344 creator A5053107465 @default.
- W2389837344 date "2012-01-01" @default.
- W2389837344 modified "2023-09-26" @default.
- W2389837344 title "The Effect of Drosophila SCS' Insulator on Transgene Expression Mediated by Baculovirus in Mammalian Cells" @default.
- W2389837344 hasPublicationYear "2012" @default.
- W2389837344 type Work @default.
- W2389837344 sameAs 2389837344 @default.
- W2389837344 citedByCount "0" @default.
- W2389837344 crossrefType "journal-article" @default.
- W2389837344 hasAuthorship W2389837344A5053107465 @default.
- W2389837344 hasConcept C100012643 @default.
- W2389837344 hasConcept C102230213 @default.
- W2389837344 hasConcept C103878061 @default.
- W2389837344 hasConcept C104317684 @default.
- W2389837344 hasConcept C142613039 @default.
- W2389837344 hasConcept C150194340 @default.
- W2389837344 hasConcept C153911025 @default.
- W2389837344 hasConcept C159047783 @default.
- W2389837344 hasConcept C2522874641 @default.
- W2389837344 hasConcept C2777773057 @default.
- W2389837344 hasConcept C39060996 @default.
- W2389837344 hasConcept C40767141 @default.
- W2389837344 hasConcept C54355233 @default.
- W2389837344 hasConcept C86803240 @default.
- W2389837344 hasConcept C92087593 @default.
- W2389837344 hasConcept C95444343 @default.
- W2389837344 hasConceptScore W2389837344C100012643 @default.
- W2389837344 hasConceptScore W2389837344C102230213 @default.
- W2389837344 hasConceptScore W2389837344C103878061 @default.
- W2389837344 hasConceptScore W2389837344C104317684 @default.
- W2389837344 hasConceptScore W2389837344C142613039 @default.
- W2389837344 hasConceptScore W2389837344C150194340 @default.
- W2389837344 hasConceptScore W2389837344C153911025 @default.
- W2389837344 hasConceptScore W2389837344C159047783 @default.
- W2389837344 hasConceptScore W2389837344C2522874641 @default.
- W2389837344 hasConceptScore W2389837344C2777773057 @default.
- W2389837344 hasConceptScore W2389837344C39060996 @default.
- W2389837344 hasConceptScore W2389837344C40767141 @default.
- W2389837344 hasConceptScore W2389837344C54355233 @default.
- W2389837344 hasConceptScore W2389837344C86803240 @default.
- W2389837344 hasConceptScore W2389837344C92087593 @default.
- W2389837344 hasConceptScore W2389837344C95444343 @default.
- W2389837344 hasLocation W23898373441 @default.
- W2389837344 hasOpenAccess W2389837344 @default.
- W2389837344 hasPrimaryLocation W23898373441 @default.
- W2389837344 hasRelatedWork W1579784411 @default.
- W2389837344 hasRelatedWork W2021897533 @default.
- W2389837344 hasRelatedWork W2100600401 @default.
- W2389837344 hasRelatedWork W2103202783 @default.
- W2389837344 hasRelatedWork W2107497648 @default.
- W2389837344 hasRelatedWork W2150770791 @default.
- W2389837344 hasRelatedWork W2351893465 @default.
- W2389837344 hasRelatedWork W2362071913 @default.
- W2389837344 hasRelatedWork W2387805344 @default.
- W2389837344 hasRelatedWork W2389157526 @default.
- W2389837344 hasRelatedWork W2392201987 @default.
- W2389837344 hasRelatedWork W2392886669 @default.
- W2389837344 hasRelatedWork W2402735344 @default.
- W2389837344 hasRelatedWork W2413565240 @default.
- W2389837344 hasRelatedWork W2414579919 @default.
- W2389837344 hasRelatedWork W2433034936 @default.
- W2389837344 hasRelatedWork W2462924477 @default.
- W2389837344 hasRelatedWork W2761425244 @default.
- W2389837344 hasRelatedWork W2982619769 @default.
- W2389837344 hasRelatedWork W650843890 @default.
- W2389837344 isParatext "false" @default.
- W2389837344 isRetracted "false" @default.
- W2389837344 magId "2389837344" @default.
- W2389837344 workType "article" @default.