Matches in SemOpenAlex for { <https://semopenalex.org/work/W2389840305> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2389840305 abstract "Objective To establish and evaluate the method using real-time fluorescence quantitative polymerase chain reaction based on TaqMan technique for detecting TNF-related apoptosis inducing ligand (TRAIL) mRNA in mononuclear cell (PBMC)of human peripheral blood.Methods The probe and specific primers (upper and lower) were designed according to the sequences of the second and the third extron of the TRAIL gene respectively.The fluorescence of PCR products was detected continuously during amplification.According to the standard curve created by plasmid DNA,the expression levels of TRAIL mRNA in clinical samples were determined with software and the results presented as the ratios of TRAIL mRNA toβ2M mRNA.Results The detection range of the assay was from 2.9×103 to 2.9×109 copies/ml. The coefficients of variation (CV) of intra-experimental and inter-experimental reproducibility were 9.5% and 16.7% respectively.The ratios of TRAIL mRNA toβ2M mRNA in patients with chronic hepatitis B were significantly higher than those of blood donors (P0.001).Conclusion The assay established in this study presented high sensitivity and reproducibility, so it is suitable for measurement of the expression level of TRAIL-mRNA" @default.
- W2389840305 created "2016-06-24" @default.
- W2389840305 creator A5013034880 @default.
- W2389840305 date "2007-01-01" @default.
- W2389840305 modified "2023-09-28" @default.
- W2389840305 title "Detection of TRAIL mRNA of mononuclear cell in peripheral blood by TaqMan real-time fluorescence quantitative RT-PCR" @default.
- W2389840305 hasPublicationYear "2007" @default.
- W2389840305 type Work @default.
- W2389840305 sameAs 2389840305 @default.
- W2389840305 citedByCount "0" @default.
- W2389840305 crossrefType "journal-article" @default.
- W2389840305 hasAuthorship W2389840305A5013034880 @default.
- W2389840305 hasConcept C104317684 @default.
- W2389840305 hasConcept C105580179 @default.
- W2389840305 hasConcept C121332964 @default.
- W2389840305 hasConcept C137061746 @default.
- W2389840305 hasConcept C143660080 @default.
- W2389840305 hasConcept C153911025 @default.
- W2389840305 hasConcept C185592680 @default.
- W2389840305 hasConcept C202751555 @default.
- W2389840305 hasConcept C43617362 @default.
- W2389840305 hasConcept C48023723 @default.
- W2389840305 hasConcept C49105822 @default.
- W2389840305 hasConcept C55493867 @default.
- W2389840305 hasConcept C62520636 @default.
- W2389840305 hasConcept C86803240 @default.
- W2389840305 hasConcept C89838059 @default.
- W2389840305 hasConcept C91881484 @default.
- W2389840305 hasConcept C9893847 @default.
- W2389840305 hasConceptScore W2389840305C104317684 @default.
- W2389840305 hasConceptScore W2389840305C105580179 @default.
- W2389840305 hasConceptScore W2389840305C121332964 @default.
- W2389840305 hasConceptScore W2389840305C137061746 @default.
- W2389840305 hasConceptScore W2389840305C143660080 @default.
- W2389840305 hasConceptScore W2389840305C153911025 @default.
- W2389840305 hasConceptScore W2389840305C185592680 @default.
- W2389840305 hasConceptScore W2389840305C202751555 @default.
- W2389840305 hasConceptScore W2389840305C43617362 @default.
- W2389840305 hasConceptScore W2389840305C48023723 @default.
- W2389840305 hasConceptScore W2389840305C49105822 @default.
- W2389840305 hasConceptScore W2389840305C55493867 @default.
- W2389840305 hasConceptScore W2389840305C62520636 @default.
- W2389840305 hasConceptScore W2389840305C86803240 @default.
- W2389840305 hasConceptScore W2389840305C89838059 @default.
- W2389840305 hasConceptScore W2389840305C91881484 @default.
- W2389840305 hasConceptScore W2389840305C9893847 @default.
- W2389840305 hasLocation W23898403051 @default.
- W2389840305 hasOpenAccess W2389840305 @default.
- W2389840305 hasPrimaryLocation W23898403051 @default.
- W2389840305 hasRelatedWork W2076653387 @default.
- W2389840305 hasRelatedWork W2130091824 @default.
- W2389840305 hasRelatedWork W2351723204 @default.
- W2389840305 hasRelatedWork W2354001115 @default.
- W2389840305 hasRelatedWork W2354641489 @default.
- W2389840305 hasRelatedWork W2358398870 @default.
- W2389840305 hasRelatedWork W2370549752 @default.
- W2389840305 hasRelatedWork W2372714824 @default.
- W2389840305 hasRelatedWork W2374576732 @default.
- W2389840305 hasRelatedWork W2374819882 @default.
- W2389840305 hasRelatedWork W2375489729 @default.
- W2389840305 hasRelatedWork W2377482635 @default.
- W2389840305 hasRelatedWork W2378669086 @default.
- W2389840305 hasRelatedWork W2381340714 @default.
- W2389840305 hasRelatedWork W2386010772 @default.
- W2389840305 hasRelatedWork W2495891243 @default.
- W2389840305 hasRelatedWork W3032100626 @default.
- W2389840305 hasRelatedWork W3144340568 @default.
- W2389840305 hasRelatedWork W3148259882 @default.
- W2389840305 hasRelatedWork W2411945864 @default.
- W2389840305 isParatext "false" @default.
- W2389840305 isRetracted "false" @default.
- W2389840305 magId "2389840305" @default.
- W2389840305 workType "article" @default.