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- W2389894013 abstract "An electrochemical immunosensor was developed for the ultrasensitive measurement of α-fetoprotein based on electrochemical stripping analysis of silver nanoparticles catalytically deposited by nanogold labels.The immunosensor was prepared by covalently immobilizing capture antibodies with glutaraldehyde(GA) on chitosan-multi wall carbon nanotubes modified glassy carbon electrodes.Through a sandwich-type immunoreaction,antibody-functionalized AuNPs were captured onto the immunosensor surface,and further induced a subsequent silver deposition.The deposited AgNPs were then measured directly by anodic stripping analysis in KCl solution.Under the optimal conditions,the calibration curve was linear over α-fetoprotein concentration in the range of 0.02-200 μg/L,the linear regression equation is ΔI=-0.13-0.11c(μg/L)(r=0.992 8).The detection limit is 8 ng/L.The electrochemical immunosensor was applied in the determination of α-fetoprotein in three serum samples.The relative standard deviation is no more than 6.1%.The determination result is identical with the result of electrogenerated chemiluminescence method.The sensor showed acceptable reproducibility,stability and accuracy,indicating a promising potential in clinical diagnose." @default.
- W2389894013 created "2016-06-24" @default.
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- W2389894013 date "2012-01-01" @default.
- W2389894013 modified "2023-09-25" @default.
- W2389894013 title "Ultrasensitive α-Fetoprotein Immunosensor Based on Electrochemical Stripping Analysis of Silver Deposition" @default.
- W2389894013 hasPublicationYear "2012" @default.
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