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- W2389904385 abstract "Objective Macroporous resin,a polymeric sorbent with a good adsorption property developed in recent years,is widely applicable to the separation and purification of active ingredients.Few researches are reported on the total flavonoids from folium callicarpae pedunculatae(FCP) at home and abroad.The authors studied the techniques of the extraction and purification of total flavonoids,aiming at increasing the content of total flavonoids in the crude extracts of FCP.Methods The extraction process was optimized by orthogonal design trial with the content of total flavonoids as the index.Macroporous resin with better adsorbability was selected for this study.And the rates of transfer and purity of total flavonoids were used as the index in the optimization of the purification process.Results The optimum conditions for the extraction of FCP were 20 times the amount of 70% alcohol and three extractions of 1.5 h each,and those for its purification with AB-8 macroporous resin were sample concentration of 3.77 mg/mL,adsorption velocity of 1 BV/h,70% alcohol as the desorption solvent,eluting velocity of 2BV/h,and eluate volume of 4BV.Conclusion After purification with AB-8 macroporous resin,the purity of total flavonoids in the extract of FCP amounted to 57%.This process can be used to effectively separate and concentrate total flavoionds from FCP." @default.
- W2389904385 created "2016-06-24" @default.
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- W2389904385 date "2011-01-01" @default.
- W2389904385 modified "2023-09-23" @default.
- W2389904385 title "Extraction and purification of total flavonoids from folium callicarpae pedunculatae with macroporous resin" @default.
- W2389904385 hasPublicationYear "2011" @default.
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