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- W2389921836 abstract "Upstream sequence and partial coding sequence(CDS) of the phosphoglycerate kinase(PGK) gene were cloned from oleaginous yeast Trichosporon sp.by degenerate PCR and chromosome walking.The computation analysis revealed that a promoter-like sequence in the upstream region of the PGK coding region including essential elements such as TATA BOX and CAAT BOX was cloned.The promoter-proximal region cloned was fused with the coding region of the hygromycin gene,and then cloned into the expression vector pTF-PHT.The recombinant vector was transformed into Trichosporon fermentans,and the transformed yeast was able to grow on the selective agar containing hygromycin.The results indicated that the promoter-proximal region of the phosphoglycerate kinase gene of Trichosporon sp was able to drive the expression of heterologous genes in the Trichosporon fermentans,which provides new avenues for developing novel engineering yeast or yeast expression system." @default.
- W2389921836 created "2016-06-24" @default.
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- W2389921836 date "2012-01-01" @default.
- W2389921836 modified "2023-09-25" @default.
- W2389921836 title "Construction of a Novel Expression Vector with the Promoter of Phosphoglycerate Kinase Gene and Its Utilization of Heterogenous Gene Expression in Trichosporon fermentans" @default.
- W2389921836 hasPublicationYear "2012" @default.
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