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- W2389931166 abstract "The eukaryotic expression vector pIRES2-PC-1-46was construc te d by inserting a599bp DNA fragment ,which consisted of the N-terminus46am ino acids sequence and its upstream untranslation region of human PC-1gene,in to the vector pIRES2.The human breast cancer cell MCF-7was transfected with pIRES2-PC-1-46using Lipofectamine regent.The positive cell clones were s elected with G418.Utilizing PCR and RT-PCR technologies,the insertion and expression of exogenous PC-1gene in MCF-7cells were analysed.The results of stable transfec-tion and high expression of exogenous PC-1gene in MCF-7cel ls were confirmed by PCR and RT-PCR.The breast cancer cell line MCF-7-PC-1-46that stably expressed PC-1gene N-terminus46amino acids was establis hed.It will facilitate further studies on biological function of the PC-1gene ." @default.
- W2389931166 created "2016-06-24" @default.
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- W2389931166 date "2003-01-01" @default.
- W2389931166 modified "2023-09-25" @default.
- W2389931166 title "Establishment of a breast cancer cell line MCF-7-PC-1-46" @default.
- W2389931166 hasPublicationYear "2003" @default.
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