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- W2389955276 abstract "This research aimed to investigate the effects of 3-Monochloropropane-1,2-diol(3 MCPD) on the cell growth in Rat Leydig tumor cells R2C.R2C cells were exposed to 0.5,1,2,4,6 mmol/L 3-Monochloropropane-1,2-diol for 48 h,different concentrations(IC25,IC50,IC75) of 3 MCPD were elected by MTT assay.Three specific concentrations of 3-MCPD were cultured with R2C cells for 4 h and 24 h,respectively.The damage of DNAs in R2C cells was investigated by single cell gel electrophoresis(SCGE).Progesterone level in the supernatant of cell culture medium was measured by radio immunoassay(RIA).Results indicated that 3-MCPD could inhibit cells growth,the IC25,IC50 and IC75 value were 1.027 mmol/L,1.802 mmol/L and 3.160 mmol/L,respectively.Compared with the control group,IC75 concentration of 3-MCPD incubated R2C cells for 4h,the damage of DNAs were obviously higher and the progesterone level were decreased 16.0%.In addition,three concentrations of 3-MCPD incubated R2C cells for 24 h,the progesterone level were obviously decreased from 9.5% to 61.2%.The study suggested that 3-MCPD could directly affect cell growth and the progesterone synthesis of R2C cells." @default.
- W2389955276 created "2016-06-24" @default.
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- W2389955276 date "2013-01-01" @default.
- W2389955276 modified "2023-09-25" @default.
- W2389955276 title "Effects of 3-Monochloropropane-1,2-Diol on the Cell Growth in Rat Leydig Tumor Cells R2C" @default.
- W2389955276 hasPublicationYear "2013" @default.
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