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- W2389969369 abstract "Pyrroloquinoline quinone( PQQ) is a water-soluble,redox-cycling orthoquinone which was found to be a cofactor for membrane-bound dehydrogenases in the bacterial system. It may be a novel vitamin because it has been shown to be essential for normal growth and development,and is present in many animal tissues. Methylotrophs can reduce one-carbon compounds as the carbon source for their growth,which was thought to be the highest-yield strain of PQQ. A large number of methanol-utilizing bacteria that can produce extracellular pyrroloquinoline quinone were screened by using methanol as the sole carbon and energy sources. A methylotroph with a low protein formation,FJNU-6,was identified as Hyphomicrobium denitrificans based on morphology,physiological and biochemical properties,together with 16 S r DNA sequence analysis. To further improve the production of pyrroloquinoline quinone by H. denitrificans FJNU-6 the fractional factorial and central composite design was applied to optimize the medium. The optimal medium for H. denitrificans FJNU-6 was Me OH 12 g / L,( NH4)2SO41. 68 g / L,KH2PO43. 01 g / L,Na2HPO49. 89 g / L,Mg SO4·7H2O 1. 1 g / L,mineral water 0. 9 m L / L,vitamin solution 1. 0 m L / L,and the optimal culture temperature is 30° C and culture time is 88 h. Under these conditions,the amount of protein in the broth was only 32. 17 mg / L and the production of pyrroloquinoline quinone increased to121. 43 mg / L,with an increase of 2. 95 fold compared with the original medium components. This study revealed that H. denitrificans FJNU-6 displayed a good potential for industrial production of pyrroloquinoline quinone,and provided the references for further study of pyrroloquinoline quinone production in the future." @default.
- W2389969369 created "2016-06-24" @default.
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- W2389969369 date "2014-01-01" @default.
- W2389969369 modified "2023-09-24" @default.
- W2389969369 title "Screening and Fermentation Optimization for A Pyrroloquinoline Quinone Producing Strain" @default.
- W2389969369 hasPublicationYear "2014" @default.
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