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- W2389981680 abstract "A pair of specific primers was designed according to gene sequences ORF7 of PRRSV VR2332 strain to amplify a complete ORF7 gene by RT-PCR.The PCR products were cloned into the vector pMD-18T and sequenced.The double-digested fragment of the sequenced recombinant plasmid pMD-18T-N was obtained and connected to prokaryotic expression vector pET-28a(+).Recombinant plasmid pET-28a(+)-N was converted to the host bacteria BL21 and the conditions(concentrations of IPTG,induction time,induction temperature) were optimized.The results showed that 37 ℃,0.2 mmol/L IPTG induced 5 h can realize high expression of the recombinant protein.Ni-NTA affinity chromatography and Western-blotting analysis showed that expression recombinant N protein can react specifically with PRRSV-positive sera." @default.
- W2389981680 created "2016-06-24" @default.
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- W2389981680 date "2011-01-01" @default.
- W2389981680 modified "2023-09-24" @default.
- W2389981680 title "Cloning,expression and identification of porcine reproductive and respiratory syndrome virus ORF7" @default.
- W2389981680 hasPublicationYear "2011" @default.
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