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- W2389998284 abstract "Objective To elevate the sensitivity of hepatitis C virus(HCV) detection for diagnosis of HCV infection and to emphasize the importance of peripheral blood mononuclear cells(PBMCs) for diagnosis and treatment of HCV infection.Methods Blood serum and PBMCs were obtained from patients possibly infected with HCV.Real-time fluorescent quantitative reverse transcription polymerase chain reaction(FQ-RT-PCR)was used to detect HCV RNA in serum and PBMCs respectively.Results In 180 patients possibly infected with HCV,106 individuals were detected with serum HCV RNA positivity or PBMCs positivity.In the 106 cases,serum HCV RNA was detected in 84 patients(79.25%) and PBMC HCV RNA was detected in 63 patients(59.43%).43 patients(40.57%) were only serum HCV RNA positive;22 patients(20.75%) were only PBMCs HCV RNA positive;41 patients(38.68%) were both serum and PBMC HCV RNA positive.HCV RNA level was higher in serum than in PBMCs,but there was no significant difference.Conclusion Detection of both serum and PBMC HCV RNA can be favor for diagnosis and treatment HCV infection and especially PBMCs HCV RNA is also important to assess the virologic response to interferon treatment and is reference for antiviral therapy time." @default.
- W2389998284 created "2016-06-24" @default.
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- W2389998284 date "2006-01-01" @default.
- W2389998284 modified "2023-09-24" @default.
- W2389998284 title "Detection of hepatitis C virus in serum and peripheral blood mononuclear cells with method of FQ-RT-PCR" @default.
- W2389998284 hasPublicationYear "2006" @default.
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