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- W2389999723 abstract "Objective:To compare the growth characteristics and differentiation into neuron-like cells of mesenchymal stem cells (MSCs) derived from rat fetal blood with MNCs from rat bone marrow in vitro.Method:Individual mononuclear cells (MNCs) were isolated using standard Ficoll-hypague techniques from rat fetal blood and bone marrow and then cultured in DMEM/LG containing 10% fetal bovine serum (FBS). The immunophenotype of MSCs were studied by flow cytometry. The expanded MSCs were induced to differentiate into neurons-like cells by β-mercaptoethanol (β-ME)、dimethylsulfoxide (DMSO)、butylated hydroxyanisole (BHA). The specific markers of these neuron-like cells were detected by immunocytochemistry.Result:The obvious difference was not showed in morphology and immunophenotype between two kinds of MSCs. The induced cells expressed Nestin and neuron-specific enolase(NSE), but not glial fibrillary acidic protein (GFAP).Conclusion:The evident difference is not found in biological characteristics and ability of differentiation into neuron-like cells between rat fetal blood and bone marrow-derived MSCs. So fetal blood might be another resource of MSCs." @default.
- W2389999723 created "2016-06-24" @default.
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- W2389999723 date "2006-01-01" @default.
- W2389999723 modified "2023-09-27" @default.
- W2389999723 title "Differentiating ability of mesenchymal stem cells from rat fetal blood and bone marrow in vitro" @default.
- W2389999723 hasPublicationYear "2006" @default.
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