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- W2390053283 abstract "Objective Specifity and sensitivity of a PCR assay of detecting Brucella DNA with th ree pair primers of cod-ing Br.abortus one and the same prote in gene sequence of different amplic ation fragment (that is 330bp?230bp and 223bp)were studied.Mothod DNA were extracted with boilling and phenol from 5species Brucella,Y.en terocolitica 03and 09,E.coli O:157and PCR was tested.Results As little as 1pg of Brucella DNA was detected by this mothod.DNA from the re presentative strains of five species were analyze d and yielded exclusively relevant f ragment.No amplification was detected with DNA form 2gram -negative bacteria showing serological cross -reactions with Brucella spp.Conclusion The PCR of 3pair primers were good specifity and sensitivity,but the stability of P 5 and P 6 primers was a little badness." @default.
- W2390053283 created "2016-06-24" @default.
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- W2390053283 date "2002-01-01" @default.
- W2390053283 modified "2023-09-25" @default.
- W2390053283 title "A study of detecting Brucella DNA by PCR" @default.
- W2390053283 hasPublicationYear "2002" @default.
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