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- W2390065860 abstract "The assay was aim to express swine hepatitis E virus(sHEV) ORF2partial gene fragments,purify the expression product and analyze the antigenicity.ORF2gene was amplified by PCR from sHEV cDNA as a template,and the recombinant plasmid pET32a-dORF2was constructed,then transformed into the expression bacteria BL21.After IPTG induction,the protein was analyzed by SDS-PAGE and Western blotting.The results showed that the protein was expressed fromORF2gene in the form of soluble,the protein was 60ku.The result of Western blotting analysis indicated that the protein could react with the HEV positive serum." @default.
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- W2390065860 date "2012-01-01" @default.
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- W2390065860 title "Swine Hepatitis E Virus ORF2 Partial Gene Expression and Identification of Prokaryotic" @default.
- W2390065860 hasPublicationYear "2012" @default.
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