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- W2390066644 abstract "To prepare the monoclonal antibody(MAb) against VP6 protein of group A porcine rotavirus(RV) and identify the antigenic epitope, a hybridoma stable secreting MAb against VP6 protein was prepared by fusing SP2/0 cells with spleen cells from BALB/c mice immunized with recombinant VP6 protein. Identification of the MAb indicated that it belonged to Ig G1 subtype withκ chain. The titers in cell culture medium of the hybridoma and ascites were 1 ∶12 800 and 1 ∶106, respectively. In addition, the MAb was positively reacted with authentic VP6 protein of porcine RV detected by western blot and indirect immunofluoresence assay. Moreover, the antigenic epitope sequence of134DYIENWNLQNR144 was identified by the pepscan technique. Those results provided a basis for the further study on the function of VP6 and development of diagnostic method." @default.
- W2390066644 created "2016-06-24" @default.
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- W2390066644 date "2014-01-01" @default.
- W2390066644 modified "2023-09-24" @default.
- W2390066644 title "Preparation of the monoclonal antibody against VP6 in group A porcine rotavirus and its antigenic epitope identification" @default.
- W2390066644 hasPublicationYear "2014" @default.
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