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- W2390085864 abstract "【Objective】 A HPLC method with variable wavelength detector for simultaneous determination of four synthetic colorants not permitted to add into wine by national standards,namely tartrazine,sunset yellow,carmine and amaranth,was developed to give reference for detection of synthetic colorants in wine.【Method】 Wine samples were pretreated by liquid-liquid extraction,polyamide absorption,and direct injection;the detection conditions of a HPLC method with variable wavelength detector were then optimized,and the recovery of standard addition and the precision were also tested by the developed method.【Result】 Wine samples were pretreated by polyamide absorption.The optical chromatographic conditions:5 μm particle size reversed phase column Agilent ZORBAX SB-C18(4.6 mm×250 mm) was used and the mobile phase consisted of solution A(methanol) and solution B(ammonium acetate 0.02 mol/L) with gradient elution and variable wavelength detection,and the detection wavelengths for tartrazine,sunset yellow,carmine and amaranth were 430 nm(0-2.2 min),230 nm(2.50-3.95 min),480 nm(3.95-5.00 min),480 nm(3.95-5.00 min),respectively.The flow rate was 1.0 mL/min,column temperature 35 ℃,detection time 5 min,and the injection volume 20 μL.When the four standards of the colorants were added into the wine respectively,the average relative standard deviation(RSD) was 0.73%-1.51%,and the average recovery 87.83%-107.42%.【Conclusion】 A HPLC method,with variable wavelength detector developed to simultaneously determine four synthetic colorants not permitted in wine,is a simple,fast,reliable and reproducible one." @default.
- W2390085864 created "2016-06-24" @default.
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- W2390085864 date "2011-01-01" @default.
- W2390085864 modified "2023-09-23" @default.
- W2390085864 title "Simultaneous determination of four synthetic colorants in wine by a HPLC method with variable wavelength detector" @default.
- W2390085864 hasPublicationYear "2011" @default.
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