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- W2390096156 abstract "Firstly design and synthesize four pairs of oligonucleotide sequence target myostatin,and synthesize high-expression vector.Transfect shRNA and high-expression vector into HEK293 cells,the gene silencing efficacy of the four targets was compared by real-time PCR,the most effective shRNA expression vector screened and vector pDONR221 executed BP recombination reaction,so to obtain an entry vector containing interference sequence.Then the entry vector containing interference sequence and the pLenti6/BLOCK-iT-DEST vector executed LR recombination reaction to get the lentiviral expression vector with interference sequence.The physical titer of the virus was analyzed by qPCR detection.Successfully constructed lentivector-mediated-shRNA,and the titer was 9.3×109TU/mL.Provide a stable infected cells vector to study the impact of myostatin gene on muscle growth." @default.
- W2390096156 created "2016-06-24" @default.
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- W2390096156 date "2012-01-01" @default.
- W2390096156 modified "2023-09-28" @default.
- W2390096156 title "Construction and identification of lentivial RNA interference vector of sheep myostatin receptor gene" @default.
- W2390096156 hasPublicationYear "2012" @default.
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