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- W2390133698 abstract "Objective To study the effects of dihydrotestosterone( DHT) on the expression of GDNF and the changes of signaling pathway in primary cultured rat Sertoli cells. Methods The primary cultured 20 days old sertoli cells stimulateused by DHT,immumofluorescence was used to detect the localization and expression of GDNF in Sertoli cell,Western blot was used to detect the expressions of GDNF,ERK1 /2 and CREB,and the phosphorylations of ERK1 /2 and CREB. while the inhibitors of ERK and c AMP / PKA signaling pathway were examined.Results GDNF expressed in cytoplasm of Sertoli cell. DHT promoted the expression of GDNF,while the level of ERK1 /2 and CREB phosphorylation were increased. U0126 and H89 decreased the DHT-induced GDNF expression. Conclusions DHT stimulated the expression of GDNF of Setoli cell by activing the ERK signaling pathway." @default.
- W2390133698 created "2016-06-24" @default.
- W2390133698 creator A5071390648 @default.
- W2390133698 date "2015-01-01" @default.
- W2390133698 modified "2023-09-25" @default.
- W2390133698 title "Dihydrotestosterone regulates GDNF expression in primary cultured rat Sertoli cells" @default.
- W2390133698 hasPublicationYear "2015" @default.
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