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- W2390143127 abstract "【Objective】The objectives of this experiment were to clone insulin-induced gene 1(INSIG1)sequence of Saanen Dairy Goat from Northwest AF University,and to analyze the sequence characterization and quantitative tissues expression.【Method】The coding sequence region was cloned from goats mammary gland total RNA by PCR reaction with primers designed based on bovine INSIG-1,and sequence characterization was performed by biology information analyzing software.INSIG-1 gene expression patterns in ten tissues(subcutaneous fat,muscles,mammary gland,liver,kidney,heart,lung,spleen,rumen and small intestine)were conducted using real-time fluorescence quantitative PCR(RT-qPCR).【Result】The experiment obtained a 1 014bp sequence(GenBank,accession number JQ665439),which contained a coding sequence region of 831bp and 276 amino acids with molecular weight of 29.70ku and pI of 8.99.The genetic distance analysis showed that goat had the closest relationship with bovine(Bos taurus).Nucleotides and amino acid sequences alignment showed both the nucleotides and amino acid of goat INSIG-1 had the highest similarity score(97%and 90%,respectively) with bovine in GenBank.Protein structure analysis of goat INSIG-1 protein showed that INSIG-1 contained five transmembranehelices and hydrophobicity analysis showed that the protein was hydrophilic.INSIG-1 expression patterns were examined by RT-qPCR,and the results showed that the liver had the highest expression level,followed by fat,and mammary gland while heart had the lowest expression level.【Conclusion】This study cloned originally INSIG-1 gene coding sequence region of Saanen Dairy Goat from Northwest AF University,and found the gene expression patterns." @default.
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- W2390143127 date "2013-01-01" @default.
- W2390143127 modified "2023-09-27" @default.
- W2390143127 title "Cloning, sequence characters and tissue expression pattern of INSIG-1 in Saanen Dairy Goat." @default.
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