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- W2390153582 abstract "Most benthic marine invertebrates have pelagic larvae that develop to competent stage and then settle on the bottom in response to physical,biological and chemical factors.Many previous investigators focused on exploring the inductive effects of chemical cues on larval settlement and metamorphosis.In this study,a ORF of epidermal growth factor receptor(EGFR) was amplified from sengmao oyster,Crassostrea gigas angulata and the target gene was cloned into prokaryotic expressing vector,pET-32a.After transformation,a soluble protein fused with a hexahistidine affinity ligand was expressed in E.coli BL21(DE3).The 50 ku recombinant protein was highly purified using affinity chromatography.As demonstrated by RT-PCR,EGFR was strongly expressed after 1 to 6 h of attachment of sengmao oyster larvae and weekly expressed before attachment and after 24 h of metamorphosis.And the result of RT-PCR indicated that EGFR played an important role during attachment and metamorphosis of sengmao oyster larvae." @default.
- W2390153582 created "2016-06-24" @default.
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- W2390153582 date "2012-01-01" @default.
- W2390153582 modified "2023-09-26" @default.
- W2390153582 title "Prokaryotic Expression and mRNA Expression Analysis of Epidermal Growth Factor Receptor During Attachment and Metamorphosis from Crassostrea gigas angulata" @default.
- W2390153582 hasPublicationYear "2012" @default.
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