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- W2390167600 abstract "AIM: To investigate the mechanism that nicotine inhibits perforin/granzyme B-induced apoptosis in A549 lung cancer cells.METHODS: MTT assay,acridine orange(AO)/ethidium bromide(EB)staining,Annexin V-FITC/PI staining and RT-PCR were applied to identify the viability,the morphological characteristics of apoptosis,the apoptotic rate and the mRNA expression of X-linked inhibitor of apoptosis protein(XIAP) and survivin,respectively.RESULTS: No significant apoptosis was observed when nicotine was used alone,and the proliferation of A549 lung cancer cells was inhibited by nicotine in a concentration-and time-dependent manner(P0.01).The positive rate of PI staining in A549 cells treated with 0.125 mg/L perforin was up to 90%.When 1 μL of granzyme B(1 mg/L) was added to the cells for 6 h,more than 90% apoptotic cells were found.Combination of nicotine(6 μmol/L) with perforin/granzyme B for 6 h significantly inhibited apoptosis.Furthermore,higher XIAP and survivin mRNA was detected when used nicotine alone,and the lowest XIAP and survivin mRNA was measured when used perforin/granzyme B alone.However,the highest XIAP and survivin mRNA was observed when used perforin/granzyme B combined with nicotine.CONCLUSION: Nicotine inhibits perforin/granzyme B-induced apoptosis in A549 lung cancer cells.This effect may be related to up-regulation of the inhibitor of apoptosis proteins,XIAP and survivin." @default.
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- W2390167600 date "2011-01-01" @default.
- W2390167600 modified "2023-09-22" @default.
- W2390167600 title "Nicotine inhibits perforin/granzyme B-induced apoptosis in A549 lung cancer cells" @default.
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