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- W2390170610 abstract "Objective To obtain DNA sequence of Schistosoma japonicum (Sj) arginase (ARG) novel gene and its promoter and to identify the completeness of their CDS. Methods Prepare DNA from Schistosoma japonicum adult worms, then amplify the corresponding sequences of DNA by using the same primers for amplifying CDS. Compare DNA with cDNA sequences by ClustalW in EBI to find out if there were introns among the DNA sequences. Amplify the promoter sequence of ARG gene by using TaKaRa LA Taq PCR Cloning in Vitro Kit, then analyze the position of TATA box. Results DNA sequences of Sj ARG gene was amplified from DNA of Sj adult worms. Comparing the cDNA sequences with the DNA sequences of Sj ARG showed that the two sequences of Sj ARG had no difference. It was extended for 69bp from amplifying promoter sequence by TaKaRa LA Taq PCR Cloning in Vitro Kit. There existed a sequence of 32bp before initiation codon ATG named, TATA box. The real ORF length of ARG gene should be 1 164bp. Conclusion The DNA sequence encoding Sj arginase was firstly sequenced and proved to have no intron. As the ARG gene's promoter sequence was amplified in this study, the full cDNA length of the arginase was obtained for further study." @default.
- W2390170610 created "2016-06-24" @default.
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- W2390170610 date "2005-01-01" @default.
- W2390170610 modified "2023-09-23" @default.
- W2390170610 title "Amplification and Sequence Analysis of Schistosoma japonicum Arginase Novel Gene's Promoter Sequence" @default.
- W2390170610 hasPublicationYear "2005" @default.
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