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- W2390172913 abstract "Objective To construct an eukaryotic expression vector encoding antimicrobial peptide PR39,and observe its expression and antimicrobial function in mouse macrophage RAW264.7 cells.Methods PR39 gene sequence was synthesized with PCR amplification and inserted into an eukaryotic expression vector pIRES2-GFP.After identification,pIRES2-GFP/PR39 vector was transfected into RAW264.7 cells with cationic liposome.The expression of PR39 was detected by RT-PCR and immunofluorescence method,and its antibacterial activity was also detected.Results The pIRES2-GFP/PR39 vector was successfully constructed,and PR39 was expressed in RAW 264.7 cells with antimicrobial activity.RAW 264.7 cells expressing PR39 showed stronger antibacterial ability.Conclusion Antimicrobial peptide PR39 is expressed and shows antimicrobial function in RAW264.7 cells.The successful construction of the PR39 expressing vector have established a foundation for the gene therapy of antimicrobial peptides against intracellular bacterial infection." @default.
- W2390172913 created "2016-06-24" @default.
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- W2390172913 date "2008-01-01" @default.
- W2390172913 modified "2023-09-23" @default.
- W2390172913 title "Construction of eukaryotic expression vector encoding antimicrobial peptides PR39 and its expression and antimicrobial function in RAW264.7 cells" @default.
- W2390172913 hasPublicationYear "2008" @default.
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