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- W2390185436 abstract "The aim of this study is to develop a s PCR method for the specific and sensitive identification of Mycoplasma suis.The genes encoding 16S rRNA,50S rRNA and membrane protein OxaA were used as detection targets.The results showed that PCR methods based on OxaA and 16S rRNA genes had higher sensitivity and the minimum detectable concentration was 1.86fg/μL.However,the minimum detectable concentration of 50S rRNA gene was 18.6fg/μL.The genes of E.coli,Streptococcus,M.hyopneumoniae and M.wenyonii could not be amplified by the above PCR methods.The detection results for 60 clinical samples showed that the PCR method based on OxaA gene(15/60) had significantly higher detection rate than that based on 16S rRNA gene(13/60) and 50S rRNA gene(11/60).The study provided a more sensitive and specific detection technique for diagnosis and epidemiological investigation of M.suis infection." @default.
- W2390185436 created "2016-06-24" @default.
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- W2390185436 date "2012-01-01" @default.
- W2390185436 modified "2023-09-28" @default.
- W2390185436 title "Comparison of PCR methods to different target genes of Mycoplasma suis" @default.
- W2390185436 hasPublicationYear "2012" @default.
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