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- W2390185972 abstract "Objective To construct different expression vectors of isoform cDNA aiming at MBP,taking pEGFP-N1 plasmid as mediation.Methods Three different MBP cDNA sequences were cloned into pEGFP-N1 plasmid and transformed into DH5α competent cells,which were identified and screened by restriction enzyme analysis.Results The sequencing results showed that the sequences of three inserted purposed segments were the same as theory sequences.Conclusion We construct three recombinant eukaryotic expression vectors successfully:pEGFP-N1-MBP21.5,pEGFP-N1-MBP18.5 and pEGFP-N1-MBP17.3,which lay a foundation for the further study of the expression and functional differences of different MBP cDNA in eukaryotic cells." @default.
- W2390185972 created "2016-06-24" @default.
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- W2390185972 date "2012-01-01" @default.
- W2390185972 modified "2023-09-23" @default.
- W2390185972 title "Construction and identification of eukaryotic expression vector of different cDNA sequences of MBP" @default.
- W2390185972 hasPublicationYear "2012" @default.
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