Matches in SemOpenAlex for { <https://semopenalex.org/work/W2390207989> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2390207989 abstract "The cryptogein gene was obtained by PCR amplification from genomic DNA of Phytophthora cryptogea (Fig.1). The lysine in site 13 of cryptogein protein was mutated to valine (K13V) through PCR site-directed mutagenesis. The mutant fragment (CryK13V) was confirmed by enzyme digestion and DNA sequencing. We expressed this CryK13V gene under the control of the 35S promoter of cauliflower mosaic virus (CaMV). Then CryK13V gene was fused to the signal peptide sequence of the extracellular pathogenesis-related PR1b protein of tobacco (Fig.2). The chimeric gene was transformed into tobacco by Agrobacterium-mediated transformation method, and 33 kanamycin-resistant plants were obtained. The presence of the transgene in the regenerated plants was confirmed by PCR (Fig.4) and Southern blot analysis (Fig.5). A very low level of CryK13V expression in transgenic plants could activate the expressions of defense-related gene, PR1 and OPBP1 (Fig.7) and the transgenic plants exhibited enhanced resistance to multiple pathogens such as blank shank fungus Phytophthora parasitica var. nicotiana, brown spot fungus Alternaria alternata and wild fire bacterium Pseudomonas syringae pv. tabaci (Fig.6). Our data suggest that the lysine residue at position 13 of cryptogein plays a key role in the induction of hypersensitive cell death, and analysis of cell death and the other defense responses are of potential in genetic manipulation of broad-spectrum resistance against plant pathogens." @default.
- W2390207989 created "2016-06-24" @default.
- W2390207989 creator A5056887820 @default.
- W2390207989 date "2002-01-01" @default.
- W2390207989 modified "2023-09-25" @default.
- W2390207989 title "Site-Directed Mutagenesis of Cryptogein Gene(CryK13V)and Generation of Transgenic Tobacco Plants with Nonspecific Disease Resistance" @default.
- W2390207989 hasPublicationYear "2002" @default.
- W2390207989 type Work @default.
- W2390207989 sameAs 2390207989 @default.
- W2390207989 citedByCount "0" @default.
- W2390207989 crossrefType "journal-article" @default.
- W2390207989 hasAuthorship W2390207989A5056887820 @default.
- W2390207989 hasConcept C102230213 @default.
- W2390207989 hasConcept C104317684 @default.
- W2390207989 hasConcept C122172344 @default.
- W2390207989 hasConcept C144707691 @default.
- W2390207989 hasConcept C153911025 @default.
- W2390207989 hasConcept C200148052 @default.
- W2390207989 hasConcept C204241405 @default.
- W2390207989 hasConcept C2522874641 @default.
- W2390207989 hasConcept C2779656422 @default.
- W2390207989 hasConcept C2780037811 @default.
- W2390207989 hasConcept C2780381237 @default.
- W2390207989 hasConcept C2781205006 @default.
- W2390207989 hasConcept C54355233 @default.
- W2390207989 hasConcept C86803240 @default.
- W2390207989 hasConcept C93678976 @default.
- W2390207989 hasConceptScore W2390207989C102230213 @default.
- W2390207989 hasConceptScore W2390207989C104317684 @default.
- W2390207989 hasConceptScore W2390207989C122172344 @default.
- W2390207989 hasConceptScore W2390207989C144707691 @default.
- W2390207989 hasConceptScore W2390207989C153911025 @default.
- W2390207989 hasConceptScore W2390207989C200148052 @default.
- W2390207989 hasConceptScore W2390207989C204241405 @default.
- W2390207989 hasConceptScore W2390207989C2522874641 @default.
- W2390207989 hasConceptScore W2390207989C2779656422 @default.
- W2390207989 hasConceptScore W2390207989C2780037811 @default.
- W2390207989 hasConceptScore W2390207989C2780381237 @default.
- W2390207989 hasConceptScore W2390207989C2781205006 @default.
- W2390207989 hasConceptScore W2390207989C54355233 @default.
- W2390207989 hasConceptScore W2390207989C86803240 @default.
- W2390207989 hasConceptScore W2390207989C93678976 @default.
- W2390207989 hasLocation W23902079891 @default.
- W2390207989 hasOpenAccess W2390207989 @default.
- W2390207989 hasPrimaryLocation W23902079891 @default.
- W2390207989 hasRelatedWork W1520177546 @default.
- W2390207989 hasRelatedWork W1982404912 @default.
- W2390207989 hasRelatedWork W1993792452 @default.
- W2390207989 hasRelatedWork W1996216248 @default.
- W2390207989 hasRelatedWork W2000092215 @default.
- W2390207989 hasRelatedWork W2006639378 @default.
- W2390207989 hasRelatedWork W2015373426 @default.
- W2390207989 hasRelatedWork W2057292990 @default.
- W2390207989 hasRelatedWork W2058089355 @default.
- W2390207989 hasRelatedWork W2060789164 @default.
- W2390207989 hasRelatedWork W2070482556 @default.
- W2390207989 hasRelatedWork W2088373845 @default.
- W2390207989 hasRelatedWork W2142511895 @default.
- W2390207989 hasRelatedWork W2149569026 @default.
- W2390207989 hasRelatedWork W2159814312 @default.
- W2390207989 hasRelatedWork W2241890156 @default.
- W2390207989 hasRelatedWork W2406418289 @default.
- W2390207989 hasRelatedWork W2470748485 @default.
- W2390207989 hasRelatedWork W2872257289 @default.
- W2390207989 hasRelatedWork W785567566 @default.
- W2390207989 isParatext "false" @default.
- W2390207989 isRetracted "false" @default.
- W2390207989 magId "2390207989" @default.
- W2390207989 workType "article" @default.