Matches in SemOpenAlex for { <https://semopenalex.org/work/W2390220455> ?p ?o ?g. }
Showing items 1 to 53 of
53
with 100 items per page.
- W2390220455 abstract "Objective: To investigate the key factors and parameters of the in vitro cytotoxicity test(MTT assay and LDH assay) for assessing nanomaterials referring to ASTM,so that to provide evidences for establishing standards of nanomaterial’s cytotoxicity test.Methods: The detection wavelength of MTT assay and LDH assay were obtained by full-wavelength scanning;the concentration and storage condition of the positive control APAP were tested;the experimental factors of MTT assay were tested by changing the MTT media with or without glycine buffer after adding DMSO;the experimental conditions of LDH assay were measured by changing the concentration of serum(10%,5%,2.5%) in cell culture media.The cytotoxicity of the silver nanoparticle containing hydrogel was assessed by refined MTT assay and LDH assay.Results: The double-wavelength was found at 540-570 /680 nm and 490 /680 nm,which is suitable for MTT assay and LDH assay respectively,in this experimental system.The proper concentration of positive control APAP was 20-30 mmol.L-1,which should be stored at-20 ℃ and will be stable for 14 days.The serum contained in the cell culture media could affect the LDH test with a serum concentration-depended manner,but this kind of interference could be deducted by the blank control.The IC50 of silver nanoparticle-containing hydrogel tested by refined MTT assay and LDH assay were 26.8 mg.mL-1 and 61.8 mg.mL-1,respectively.Conclusions: Two assessment points can be obtained in one experiment by combining re-fined MTT assay and LDH assay,so that the cytotoxicity of nanomaterials can be evaluated more comprehensively.In addition,this study defines the key factors and parameters of the cytotoxicity test(MTT assay and LDH assay) for assessing nanomaterials,and improves the feasibility of the method." @default.
- W2390220455 created "2016-06-24" @default.
- W2390220455 creator A5055320676 @default.
- W2390220455 date "2013-01-01" @default.
- W2390220455 modified "2023-09-22" @default.
- W2390220455 title "Study on key parameters of the in vitro cytotoxicity test in nanomaterials assessment" @default.
- W2390220455 hasPublicationYear "2013" @default.
- W2390220455 type Work @default.
- W2390220455 sameAs 2390220455 @default.
- W2390220455 citedByCount "0" @default.
- W2390220455 crossrefType "journal-article" @default.
- W2390220455 hasAuthorship W2390220455A5055320676 @default.
- W2390220455 hasConcept C109316439 @default.
- W2390220455 hasConcept C185592680 @default.
- W2390220455 hasConcept C202751555 @default.
- W2390220455 hasConcept C2777752497 @default.
- W2390220455 hasConcept C2780783641 @default.
- W2390220455 hasConcept C43617362 @default.
- W2390220455 hasConcept C55493867 @default.
- W2390220455 hasConceptScore W2390220455C109316439 @default.
- W2390220455 hasConceptScore W2390220455C185592680 @default.
- W2390220455 hasConceptScore W2390220455C202751555 @default.
- W2390220455 hasConceptScore W2390220455C2777752497 @default.
- W2390220455 hasConceptScore W2390220455C2780783641 @default.
- W2390220455 hasConceptScore W2390220455C43617362 @default.
- W2390220455 hasConceptScore W2390220455C55493867 @default.
- W2390220455 hasLocation W23902204551 @default.
- W2390220455 hasOpenAccess W2390220455 @default.
- W2390220455 hasPrimaryLocation W23902204551 @default.
- W2390220455 hasRelatedWork W1995129984 @default.
- W2390220455 hasRelatedWork W1996485059 @default.
- W2390220455 hasRelatedWork W2022390147 @default.
- W2390220455 hasRelatedWork W2092913272 @default.
- W2390220455 hasRelatedWork W2135750582 @default.
- W2390220455 hasRelatedWork W2188839523 @default.
- W2390220455 hasRelatedWork W2253622406 @default.
- W2390220455 hasRelatedWork W2284056687 @default.
- W2390220455 hasRelatedWork W2341769195 @default.
- W2390220455 hasRelatedWork W2357779852 @default.
- W2390220455 hasRelatedWork W2359167956 @default.
- W2390220455 hasRelatedWork W2365683908 @default.
- W2390220455 hasRelatedWork W2368304484 @default.
- W2390220455 hasRelatedWork W2380850110 @default.
- W2390220455 hasRelatedWork W2384930212 @default.
- W2390220455 hasRelatedWork W2413105315 @default.
- W2390220455 hasRelatedWork W2777060978 @default.
- W2390220455 hasRelatedWork W3030667680 @default.
- W2390220455 hasRelatedWork W3083080663 @default.
- W2390220455 hasRelatedWork W3149335538 @default.
- W2390220455 isParatext "false" @default.
- W2390220455 isRetracted "false" @default.
- W2390220455 magId "2390220455" @default.
- W2390220455 workType "article" @default.