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- W2390237551 abstract "The recombined plasmid pMD-ORF7 was constructed and used as standard positive template.And the FQ-PCR assay was constructed by quantitative concentration of serial 10 fold dilutions of pMD-ORF7 cDNA by optimizing circulation parameters.A standard curve was achieved.The result showed the sensitivity of this method was 1.0×100copy/μl.To detect three different samples by FQ-PCR repeatedly,the average circle threshold value was 0.14,0.14 and 0.44,with the coefficient of variation of 0.01%,0.01% and 0.02%,respectively.The FQ-PCR was proved to be with high sensitivity and repeatability.The detection of PRRSV in positive samples by both FQ-PCR and conventional RT-PCR was 100%,but the sensitivity of the former was 100 times higher than that of the latter." @default.
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- W2390237551 date "2012-01-01" @default.
- W2390237551 modified "2023-09-24" @default.
- W2390237551 title "Establishment of Fluorescent Quantitative PCR Assay by TaqMan Probe Approach for PRRSV Detection" @default.
- W2390237551 hasPublicationYear "2012" @default.
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