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- W2390246255 abstract "Objective To study the damage of human umbilical vein endothelial cells induced by ethanol and whether its injuring mechanism is related to the signal pathway of ADMA/NOS/NO. Methods HUVECs-12 was cultured in DMEM containing 10% FCS.At 80% confluence,cells were washed with D-Hank's fluid and cultured in DMEM containing 1% FCS for 24h.Cells were incubated in the presence or absence(control) of ethanol. To test the dose-effect of ethanol-induced endothelial cells damage,the cells were treated with ethanol at the doses of 25,50,100,and 200mmol/L for 24h.And the time-effect was also tested by culturing cells with ethanol at the dose of 100mmol/L for 0,3,6,12,24,and 48 h respectively.To explore the injuring feature of ethanol on endothelial cells,hydrogen peroxide(500μmol/L),a potent free radical,was selected as a positive control;vitamin C(100mg/L),an anti-oxidant medicine,was selected as a protective group.The cell morphology and viability of HUVECs-12(MTT assay) were observed to evaluate the injuring feature of ethanol.The levels of malonaldehyde(MDA),asymmetric dimethylarginine(ADMA), nitric oxide(NO),and nitric oxide synthase(NOS) activity were detected to elucidate the injuring mechanism of ethanol. Results The cell morphology and viability were changed conspicuously by ethanol at the final concentration 50～200mmol/L(OD: 0.91±0.10 ～ 0.58±0.04,inhibition ratio of cells' growth: 0.00±0.00～35.57±3.13,P0.01).And with concentration up(50～200mmol/L),the levels of MDA stepped up significantly versus vitamin C protective group(258.72±7.36～524.07±8.25,P0.01).The activity of eNOS and the activity of total NOS were descended(6.27±0.10～0.47±0.23,P0.05; 10.69±0.54～3.77±0.32,P0.05).The synthesis of NO was also inhibited significantly(191.61±7.96～88.38±5.07,P0.05).All the biological effect we have tested were time-related and the statistical significance was also conspicuous between every two ethanol treated groups(P0.05 or P0.01).The level of ADMA was the highest in 100mmol/L ethanol treated group and the peak value appeared after 24 h. Conclusions Ethanol at the concentration of 50～200mmol/L can induce significant damage on human umbilical vein endothelial cells,and ADMA plays an important role in this progress." @default.
- W2390246255 created "2016-06-24" @default.
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- W2390246255 date "2008-01-01" @default.
- W2390246255 modified "2023-09-23" @default.
- W2390246255 title "Damage of Human Umbilical Vein Endothelial Cells Induced by Ethanol and Whether Its Injuring Mechanism is Related to the Signal Pathway of ADMA/NOS/NO" @default.
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