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- W2390255554 abstract "Objective To observe whether Xuefu Zhuyu Decoction(XZD)could induce the differentiation of mesenchymal stem cells(MSCs)into cardiac myoid cells,thus seeking for safe and effective inducers.Methods The serum pharmacological method was used to induce.XZD containing serum was prepared.MSCs were isolated and cultured.The serum cytotoxicity was detected by MTT.The third generation of favorably grown cells was selected in this experiment.Cells were divided into three groups,i.e.,the vehicle control group,the XZD containing serum induced group,and the 5-azacytidine induced group.Expressions of Desmin andα-actin were detected by immunocytochemical staining method.Results Before induction protein expressions of Desmin andα-actin were negative,and few was weakly positive.There was no statistical difference in the weak positive expression rate among the 3 groups(P 0.05).After induction protein expressions of Desmin andα-actin were negative,and few was weakly positive in the vehicle control group.Protein expressions of Desmin andα-actin were positive in the XZC containing serum induced group and the 5-azacytidine induced group.There was statistical difference in the positive expression rate when compared with the vehicle control group(P0.05).Conclusions XZD played a role in in vitroinducing differentiation MSCs to cardiac myoid cells.It might participate in expressions of Desmin andα-actin." @default.
- W2390255554 created "2016-06-24" @default.
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- W2390255554 date "2013-01-01" @default.
- W2390255554 modified "2023-09-28" @default.
- W2390255554 title "Xuefu Zhuyu Decoction Containing Serumin vitroInduced Expressions of Desmin andα-actin:an Experimental Research" @default.
- W2390255554 hasPublicationYear "2013" @default.
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