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- W2390287523 abstract "A RNA aptamer modified to DNA(N1) that could bind to the target of Ni2+ was found that could bind specifically to Hg2+ ion with typical secondary structure.Thereafter,several DNA aptamers with a certain conformation of sequence mutation and splice were designed in order to increase the property of Hg2+ binding,and Aptamer-based fluorescence assay for the Hg2+ ion determination was developed.The addition of the Hg2+ to a mixture containing the duplex of a fluorophore labeled aptamer and a quencher-modified antisense nucleic acid(Q2) would force the release of Q2 from labeled aptamer,which was spontaneously accompanied by the increase of fluorescence intensity and made Hg2+in quantitative analysis.It was shown that stable labeled aptamer DNA and Q2 hydrogen bonds formed at concentration ration of 1 ∶ 3(labeled aptamer and Q2,respectively),under the condition of 5 min denaturation in 94 ℃ and 30 min renaturation in room temperature(25 ℃) in the fluorescence assay preparation.For aptamer N1,the fluorescence assay results showed that linear response toward Hg2+ concentration with fluorescence quenching system ranged from 1.25 mg/L to 20 mg/L,with the limit of detection 0.625 mg/L.In order to improve the sensitivity of aptamer N1,4 oligonucleotides(N4,N5,N6,N7) were designed and synthesized based on the structure of N1.The results showed that N5 had the best affinity and specificity to Hg2+ which displayed the linear range for the Hg2+ concentration detection was 0.156 mg/L to 2.5 mg/L with a detection limit of 78 μg/L." @default.
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- W2390287523 date "2012-01-01" @default.
- W2390287523 modified "2023-09-25" @default.
- W2390287523 title "Aptamer-Based Fluorescence Assay for Hg~(2+) Determination" @default.
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