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- W2390316529 abstract "【Objective】The insect midgut is generally lined with a unique chitin/protein structure, the peritrophic membrane (PM). PM facilitates the digestive process, and protects invertebrate digestive tracts from microbial infections. The insect intestinal mucin (ⅡM) is a major protein constitute of the PM. Therefore, PM could be potential target for developing pest control technique and the isolation and identification of ⅡM gene is necessary. 【Method】To clone and sequence the cDNA for SeⅡM, a Spodoptera exigua larval midgut cDNA expression library was constructed and screened with an anti-T.ni ⅡM antiserum. 【Result】The original cDNA expression library contained 8.51×106 recombinants and the recombination rate was 99.97%. The titer of amplified library is 2.41×109 pfu/ml. The average length of the inserted fragments was 1.9 kb by enzyme digestion. A positive cDNA clone for ⅡM, named as SeⅡM-8, was identified and sequenced. The cDNA is 2 234 bp, and the longest open reading frame codes for 714 amino acids. The similarity to Mamestra configurata intestinal mucin is 42% in amino acids sequence. It contains five chitin binding domains and one threonine-rich mucin domain. One tandem repeating unit TTTQAPTTT repeats 6 times among the mucin domainwhich is potential O-glycosylation sites. One aspartic acid-rich region contains one repeating unit, DDDKPGCNGNCPE repeats 12 times among the aspartic acid-rich region that distinguished IIMs from other insects. It is referred that there are one signal peptide and unknown CBDs in the 5′ end of SeⅡM-8. The accession number in GenBank for SeⅡM-8 is EU047712. 【Conclusion】A cDNA expression library with high quality from S. exigua midgut is constructed and a novel protein which named as SeⅡM-8 coding for ⅡM is identified in this study." @default.
- W2390316529 created "2016-06-24" @default.
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- W2390316529 date "2008-01-01" @default.
- W2390316529 modified "2023-10-08" @default.
- W2390316529 title "Molecular Cloning and Sequencing of an Insect Intestinal Mucin cDNA from Spodoptera exigua" @default.
- W2390316529 hasPublicationYear "2008" @default.
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