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- W2390323218 abstract "Objective: To appraise the feasibility of ionizing radiation induced DNA damage and repair of spleen cells in mice by pulsed-field gel electrophoresis (PFGE). Methods: Damage and repair conditions of DNA single-strand breaks (SSB) and double-strand breaks (DSB) in mice spleen cells induced by various doses of γ-ray radiation and 4 Gy γ-ray radiation were measured by PFGE, and compared with the control group. Results: The numbers of SSB and DSB in mice spleen cells induced by γ-ray radiation increased with the radiation dosage increasing, and SSB was more than DSB. DSB induced by 1Gy radiation and SSB induced by 2 Gy radiation were higher than those of the controls (t = 2.668, P 0.05; t = 5.117, P 0.01). SSB and DSB damage decreased through repair after 4 Gy radiation. The repair rate was rapid at first and most of DNA damage was repaired after 1h. The repair rate of SSB was faster than that of DSB. The percentage of SSB increased after 2 h repair time. Conclusion: The method is rapid and sensitive to determine DNA damage and repair of animal cells in vivo." @default.
- W2390323218 created "2016-06-24" @default.
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- W2390323218 date "2006-01-01" @default.
- W2390323218 modified "2023-09-26" @default.
- W2390323218 title "Detection of Ionizing Radiation Induced DNA Damage and Repair by Pulsed-Field Gel Electrophoresis" @default.
- W2390323218 hasPublicationYear "2006" @default.
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