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- W2390434194 abstract "Objective: To construct prokaryotic expression vector carrying DNA fragment encoding MYB77 of Bras-sica rapa and to express MYB77 in E.coli.Methods: The DNA fragment encoding MYB77 was obtained by RT-PCR and cloned into pGEM-T.DNA fragment with HindⅢ and EcoRⅠ sites was sequenced correctly and digested to ligate into pGEX-KG.Then the recombination vector pGEX-KG-MYB77 was transformed into E.coli Rosetta(DE3) strain to express fusion protein GST-MYB77 by IPTG induction.Bacterial bodies were disrupted by sonica-tion,and the soluble fraction of fusion proteins were purified by MagneGST protein purification system.GST-MYB77 fusion protein was verified on SDS-PAGE by Coomassie brilliant blue R250 staining and Western blotting analysis.Results: Recombinant expression plasmid pGEX-KG-MYB77 was obtained.The band of purified protein whose molecular weight was about 55.56 kD could be detected by antibody against GST.Conclusion: GST-MYB77 fusion protein was obtained,which will contribute to the study of function of MYB77 in Brassica rapa." @default.
- W2390434194 created "2016-06-24" @default.
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- W2390434194 date "2010-01-01" @default.
- W2390434194 modified "2023-09-28" @default.
- W2390434194 title "Prokaryotic Expression and Purification of the Protein MYB77 of Brassica rapa" @default.
- W2390434194 hasPublicationYear "2010" @default.
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