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- W2390436180 abstract "Objective To establish the primary culture technology and identification method for alveolar type Ⅱ epithelial (AT Ⅱ) cells.Further,to observe AT Ⅱ cells in vitro and detected the expression of SP-A.Methods The lungs were instilled with 0.08 % trypsin solution to isolate AT Ⅱ cells from lung tissue.The cell pellet was panned on an IgG-coated plastic dish at 37 °C to purify,and seeded culture plates for primary culture.The cells were identified under TEM.The purity of the culture cells was assayed with modified papanicolaou staining and the morphological changes of AT Ⅱ cells after culturing various times ( 36,72,96 ,120 h ) were observed in vitro.The growth curve was measured by MTT method.The expression of SP-A was detected by western blot.Results About 2.2×107 cells were obtained from one adult rat.The lamellar body of ATⅡ cells was observed under transmitting electron microscopy ( TEM ) and the purity of the culture cells was 90% assayed with modified papanicolaou Staining.Cells grown well and there were many granules in cytoplasm after 36 to 72 h of culture.Cells became rhomboid,the number of granules was decreased and vacuoles presented in cytoplasm after more than 96 h of culture.The expression of SP-A was abundant after 24 h to 72 h of culture and reached peak at 36 h.Conclusion 0.08 % trypsin digestion and specific immunosorption to plates coated with IgG is the efficient method to isolate,and purify AT Ⅱ cells.AT Ⅱ cells exhibit excellent growth property and the expression of SP-A is abundant after 36 to 72 h of culture and this term is good for in vitro study." @default.
- W2390436180 created "2016-06-24" @default.
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- W2390436180 date "2010-01-01" @default.
- W2390436180 modified "2023-09-28" @default.
- W2390436180 title "Isolation,purification,culture and identification of primary rat alveolar type II epithelial cells in vitro" @default.
- W2390436180 hasPublicationYear "2010" @default.
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