Matches in SemOpenAlex for { <https://semopenalex.org/work/W2390468993> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2390468993 abstract "A high-performance liquid chromatography(HPLC) method was optimized for the determination of the free amino acids and flavor-enhancer nucleotides in aquatic products. The flavor-enhancer nucleotides and amino acids of Pacific oyster(Crassostrea gigas), Farrer's scallop(Chlamys farreri) and sunray surf clam(Mactra chinensis) were determined to provide basic data for exploiting shellfish seasoning compounds. After sample preparation using purified water, free amino acids were determined by dabsyl chloride(Dabs-Cl) pre-column derivatization and reversed-phase HPLC. The best separation was obtained using a 17 mmol/L citric acid solution(pH 6.4, containing 4% dimethylformamide(DMF)) and acetonitrile(containing 4% DMF), gradient elution and a detection wavelength of 436 nm. Flavor nucleotides were determined by reversed-phase ion-pair HPLC, with a mobile phase of 0.02 mol/L KH2PO4(pH 4.5, 5 mmol/L tetramethylammonium hydroxide(TMAOH)) and acetonitrile, and a detection wavelength of 254 nm. This method displayed better precision and higher accuracy in meeting the analytical requirements. Recoveries varied from 90.2% to 108% and RSD varied from 0.7% to 7.1%. The results showed that high levels of taurine, alanine and glutamic acid were found in Pacific oyster; glycine, taurine and alanine were the principal free amino acids in Farrer's scallop and high percentages of glycine, taurine and alanine were detected in sunray surf clam. The taste activity values(TAVs) of alanine and glutamic acid in the three shellfish were 1; only the TAVs of glycine in Farrer's scallop and sunray surf clam were 1, indicating that they make a significant contribution to taste. Regarding flavor-enhancing nucleotides, Pacific oyster contained more inosine 5′-monophosphate(IMP), accounting for 80%, while Farrer's scallop contained the highest adenosine monophosphate(AMP) level followed by IMP; sunray surf clam contained IMP only. Although the concentration of flavor-enhancing nucleotides in three shellfish were low and TAVs were 1, the synergistic effect between nucleotides and amino acids has an important impact on shellfish flavor. Pacific oyster, Farrer's scallop and sunray surf clam from offshore Yantai are rich in taste, and the flavor-enhancing nucleotides and amino acids, such as taurine, are valuable for human health. The study also provides basic data for the extraction of active substances for the preparation of seafood seasoning compounds." @default.
- W2390468993 created "2016-06-24" @default.
- W2390468993 creator A5072744355 @default.
- W2390468993 date "2014-01-01" @default.
- W2390468993 modified "2023-09-24" @default.
- W2390468993 title "Determination and comparative analysis of flavor-enhancing nucleotides and amino acids in three common shellfish from offshore Yantai" @default.
- W2390468993 hasPublicationYear "2014" @default.
- W2390468993 type Work @default.
- W2390468993 sameAs 2390468993 @default.
- W2390468993 citedByCount "0" @default.
- W2390468993 crossrefType "journal-article" @default.
- W2390468993 hasAuthorship W2390468993A5072744355 @default.
- W2390468993 hasConcept C104317684 @default.
- W2390468993 hasConcept C179998833 @default.
- W2390468993 hasConcept C185592680 @default.
- W2390468993 hasConcept C2776364969 @default.
- W2390468993 hasConcept C2776845067 @default.
- W2390468993 hasConcept C2776923573 @default.
- W2390468993 hasConcept C2777756961 @default.
- W2390468993 hasConcept C2779701627 @default.
- W2390468993 hasConcept C2779856020 @default.
- W2390468993 hasConcept C2780626841 @default.
- W2390468993 hasConcept C2781047198 @default.
- W2390468993 hasConcept C43617362 @default.
- W2390468993 hasConcept C505870484 @default.
- W2390468993 hasConcept C512185932 @default.
- W2390468993 hasConcept C515207424 @default.
- W2390468993 hasConcept C55493867 @default.
- W2390468993 hasConcept C86803240 @default.
- W2390468993 hasConceptScore W2390468993C104317684 @default.
- W2390468993 hasConceptScore W2390468993C179998833 @default.
- W2390468993 hasConceptScore W2390468993C185592680 @default.
- W2390468993 hasConceptScore W2390468993C2776364969 @default.
- W2390468993 hasConceptScore W2390468993C2776845067 @default.
- W2390468993 hasConceptScore W2390468993C2776923573 @default.
- W2390468993 hasConceptScore W2390468993C2777756961 @default.
- W2390468993 hasConceptScore W2390468993C2779701627 @default.
- W2390468993 hasConceptScore W2390468993C2779856020 @default.
- W2390468993 hasConceptScore W2390468993C2780626841 @default.
- W2390468993 hasConceptScore W2390468993C2781047198 @default.
- W2390468993 hasConceptScore W2390468993C43617362 @default.
- W2390468993 hasConceptScore W2390468993C505870484 @default.
- W2390468993 hasConceptScore W2390468993C512185932 @default.
- W2390468993 hasConceptScore W2390468993C515207424 @default.
- W2390468993 hasConceptScore W2390468993C55493867 @default.
- W2390468993 hasConceptScore W2390468993C86803240 @default.
- W2390468993 hasLocation W23904689931 @default.
- W2390468993 hasOpenAccess W2390468993 @default.
- W2390468993 hasPrimaryLocation W23904689931 @default.
- W2390468993 hasRelatedWork W1501990696 @default.
- W2390468993 hasRelatedWork W1679012406 @default.
- W2390468993 hasRelatedWork W2061482795 @default.
- W2390468993 hasRelatedWork W2066190924 @default.
- W2390468993 hasRelatedWork W2082505301 @default.
- W2390468993 hasRelatedWork W2107386225 @default.
- W2390468993 hasRelatedWork W2325772975 @default.
- W2390468993 hasRelatedWork W2330245073 @default.
- W2390468993 hasRelatedWork W2333480462 @default.
- W2390468993 hasRelatedWork W2360688301 @default.
- W2390468993 hasRelatedWork W2367284309 @default.
- W2390468993 hasRelatedWork W2369116199 @default.
- W2390468993 hasRelatedWork W2385867337 @default.
- W2390468993 hasRelatedWork W2393417912 @default.
- W2390468993 hasRelatedWork W2398793530 @default.
- W2390468993 hasRelatedWork W761636175 @default.
- W2390468993 hasRelatedWork W787997729 @default.
- W2390468993 hasRelatedWork W835713759 @default.
- W2390468993 hasRelatedWork W880598509 @default.
- W2390468993 hasRelatedWork W1655987491 @default.
- W2390468993 isParatext "false" @default.
- W2390468993 isRetracted "false" @default.
- W2390468993 magId "2390468993" @default.
- W2390468993 workType "article" @default.