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- W2390492883 abstract "Objective To screen for gene CDK109 from the cDNA library of the neural tube from golden hamsters subjected to hyperthermia,and to clone this gene and analyze its relativity with neural tube defects(NTDs) induced by hyperthermia.Methods With Benton-Davis hybridization,a oligonucleotide probe was used to screen the cDNA library of the neural tube from a golden hamster model subjected to hyperthermia,which was constructed in our laboratory.Then the positive phage clone was transfected into E.coli BM25.8,the plasmid was isolated and identified by restriction enzyme digestion,and the positive plasmid was sequenced and blasted.By Northern blot,expression of screened gene CDK109 was detected and reconfirmed in heat-treated and control groups of golden hamsters on pregnant day 8.5.Results The whole length of the CDK109 gene was successfully screened from the cDNA library of the neural tube from golden hamsters subjected to hyperthermia.Its expression in the heat-treated group was evidently higher than in the control group.Conclusion Increased expression of CDK109 is closely related to neural tube defects induced by hyperthermia." @default.
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- W2390492883 date "2012-01-01" @default.
- W2390492883 modified "2023-10-16" @default.
- W2390492883 title "Cloning and relativity analysis of gene CDK109 related to neural tube defects induced by hyperthermia" @default.
- W2390492883 hasPublicationYear "2012" @default.
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