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- W2390495625 abstract "Aim: To observe the expression of microtubule-assosiated protein 2(MAP2) and tau protein mRNA of cultured and un-cultured mononuclear cells(MNCs)derived from human umbilical cord blood. Methods: The isolated MNCs from umbilical cord blood samples by centrifugation, were cultured with (cytokine group) or without (control group) EGF+ bFGF at a final concentration of 20 μg/L. Morphological changes of MNCs were monitored by inverse phase-contrast microscopy. MAP2 and tau mRNA were determined by RT-PCR before and after 14 d culture. Results: The freshly isolated MNCs were small and round. After culture, some cells of cytokine group became larger and were like neuron,and there were some small cells like astrocyte in the control group. The expression of tau protein mRNA was negative, but MAP2 mR- NA was positive in uncultured cells;after culture,the expression of MAP2 and tau protein mRNA were both positive,and those of cytokine group were higher than those of control group. Conclusion: Human MNCs could express MAP2 mRNA before and after incubation, express tau mRNA only by incubation, and the combination of EGF and bFGF can upregulate tau and MAP2 mRNA expression in cultured MNCs." @default.
- W2390495625 created "2016-06-24" @default.
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- W2390495625 date "2006-01-01" @default.
- W2390495625 modified "2023-09-26" @default.
- W2390495625 title "Expression of microtubule-assosiated protein 2 and tau protein mRNA in mononuclear cells derived from human umbilical cord blood" @default.
- W2390495625 hasPublicationYear "2006" @default.
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