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- W2390501217 abstract "Objective To construct CYP1A2-silenced hepatocyte through RNA interference technology and lentiviral vector,and then to study trichlorethylene(TCE) toxicity in normal human L02 hepatocyte(L02 cell) and CYP1A2-silenced hepatocyte.Methods Short hairpin RNA(shRNA) was designed and ligated to pLKO.1-puro to construct lentiviral expression vector.L02 cells were transfected.After puromycin screening,CYP1A2-silenced cells were constructed and identified by quantitative polymerase chain reaction and western blot.Then the CYP1A2-silenced hepatocytes and L02 cells were treated at various doses of TCE for 12 hours to observe the expressions of apoptosis genes(Bcl-2,Caspase-3,Caspase-8,Caspase-9) and oncogenes(c-fos,c-myc,K-ras,p53).Results Sequencing results showed that the sequence contained in the recombinant vector was exactly the same as shRNA which was designed.CYP1A2 gene expression levels and CYP1A2 protein levels in the CYP1A2-silenced hepatocytes decreased by 75.9% and 82.4% respectively when compared with normal L02 cells(P 0.01).After TCE treatment,Bcl-2 expression levels in CYP1A2-silenced hepatocytes were significantly higher than those of L02 cells(P 0.01);while the mRNA expression levels of Caspase-3,Caspase-8,Caspase-9,c-fos,c-myc,k-ras and p53 decreased in CYP1A2-silenced hepatocytes when compared with L02 cells(P 0.05 or P 0.01).Conclusion CYP1A2-silenced reduces the activation of apoptosis genes and oncogenes of hepatocytes which were treated with TCE.CYP1A2 might play an important role in TCE metabolism in vivo." @default.
- W2390501217 created "2016-06-24" @default.
- W2390501217 creator A5056500312 @default.
- W2390501217 date "2013-01-01" @default.
- W2390501217 modified "2023-09-28" @default.
- W2390501217 title "Construction of CYP1A2-silence hepatocytes and the effects of CYP1A2-silence on trichloroethylene toxicity" @default.
- W2390501217 hasPublicationYear "2013" @default.
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