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- W2390600913 abstract "Objective To construct the recombinant adenovirus of human microsomal triglyceride transfer protein with flag and GFP(hMTP-flag)with Adeasy system.Methods RNA is from HepG2 cell.The gene of hMTP-flag was amplified by RT-PCR and nested PCR.The PCR product was verified by sequencing.The gene was inserted into the shuttle vector:plasmid:GFP-Track.The recombinant plasmid confirmed by restriction enzyme digestion was linearized by PmeI digestion and transformed into the competent cell:BJ5183 containing adenovirus backbone plasmid;Adeasy.The positive homologous recombinant was linearized with PacI digestion then was transfected into 293A cells to get the packaged adenovirus followed by large scaled virus propagation.Results The gene of hMTP-flag-GFP was verified by the sequencing.Recombinant plasmid was confirmed by restriction enzyme digestion.High level GFP expression was observed under fluorescence microscope after the 293A cells were transfected with the recombinant adenovirus plasmid.Also,the hMTP band(97KD)was identified by Western-blot.Conclusion The recombinant adenovirus of hMTP-flag-GFP was fully constructed and protein expressed successfully.Based on this,the function of lipoprotein could be further discovered." @default.
- W2390600913 created "2016-06-24" @default.
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- W2390600913 date "2008-01-01" @default.
- W2390600913 modified "2023-09-24" @default.
- W2390600913 title "Construction of Recombinant Adenovirus for Microsomal Triglyceride Transfer Protein with Flag and GFP" @default.
- W2390600913 hasPublicationYear "2008" @default.
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