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- W2390636622 abstract "Objective To investigate the integrity of sperm nuclear DNA and the content ofα-1,4-glucosidase in patients with varicocele of different degrees.Methods The semem samples from 84confirmed patients with varicocele from the department of andriatrics and reproductive centre of the First Affiliated Hospital of Guangxi Medical University were assigned into 3 groups( B,C,D) based on varicocele degree.The samples of group B,C,D contained 26,28,30 cases respectively.27 semem samples from normal fertile men were collected as control group( group A).Single cell gel electropherosis( SCGE) was applied to assess the integrity of sperm nuclear DNA.The content of α-1,4-glucosidase were detected by Nitrophenol method.Results( 1) The integrity of sperm nuclear DNA and the content of α-1,4-glucosidase in patients with varicocele were significantly lower than those in the control group( P 0.05).( 2) The integrity of sperm nuclear DNA in group B was significantly higher than that in the group C and group D( P 0.05).However,there was no significant difference in sperm nuclear DNA integrity between group C and group D.( 3) The content of α-1,4-glucosidase in group B was significantly higher than that in the group C and group D( P 0.05),and the content of α-1,4-glucosidase in group C was also significantly higher than that in the group D( P 0.05).Conclusions Varicocele might lead to the decrease of sperm DNA integrity and the content of α1,4-glucoside,and affect sperm quality,the more severe of varicocele,the greater the impact." @default.
- W2390636622 created "2016-06-24" @default.
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- W2390636622 date "2014-01-01" @default.
- W2390636622 modified "2023-09-23" @default.
- W2390636622 title "Influence of varicosity degree with varicocele on sperm nuclear DNA Integrity and α-1,4-glucosidase" @default.
- W2390636622 hasPublicationYear "2014" @default.
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