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- W2390639707 abstract "Objective To establish the detection of bloodstream infections caused by methicillin-resistant Staphylococcus aureus(MRSA) mecA gene by real-time PCR.Methods Designed mecA primers and optimized experimental conditions for fluorescence real-time PCR,and used this method to detect the 78 clinical isolates of Staphylococcus aureus,compared the results with the Fox disk diffusion method.Results Among 78 strains of Staphylococcus aureus by real-time PCR method to detect fluorescence mecA gene positive 38 strains and negative 40 strains,FOX disk diffusion method to detect MRSA 27 strains,MSSA 51 strains,,it was significantly higher than the FOX disk diffusion method(P0.01) by paired Chi-square test.Conclusion Real-time fluorescence PCR method was sensitive,specific to detection of MRSA,it was more simple and rapid." @default.
- W2390639707 created "2016-06-24" @default.
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- W2390639707 date "2011-01-01" @default.
- W2390639707 modified "2023-09-28" @default.
- W2390639707 title "Research of Real-time fluorescence PCR in the detection of bloodstream infections caused by MRSA" @default.
- W2390639707 hasPublicationYear "2011" @default.
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