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- W2390743318 abstract "Aim To Constructed prokaryotic expressing plasmid pCT-P30 containing Toxoplasma gondii major surface antigen P30 and cholera toxin A 2B subunit gene and express P30-CTA 2/B fusion protein.Methods Obtained P30 gene fragment by PCR and inserted it to plasmid pUAB024 containing cholera toxin A 2/B subunit gene,Expressed fusion protein in JM109(DE3) and detected by running SDS-PAGE and Western blotting.Results Constructed correctly the expressing plasmid.The expression products were assayed by SDS-PAGE,which showed a specific protein brand and were further confirmed by western blotting showing that the specific fusion protein contained P30.Conclusion Prokaryotic expressing plasmid pUAB024-P30 can effectively express fusion protein P30-CTA 2/B." @default.
- W2390743318 created "2016-06-24" @default.
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- W2390743318 date "2003-01-01" @default.
- W2390743318 modified "2023-09-27" @default.
- W2390743318 title "Construction and expression of toxoplasma major surface antigen P30 fusion protein with cholera toxin A_2/B in Bacillus coli" @default.
- W2390743318 hasPublicationYear "2003" @default.
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