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- W2390752816 abstract "Within five years, transgenic sheep were produced by sperm-mediated exogenous DNA transfer method combined with Artification Insemination (AI) technique. The results of PCR and Southern blot showed that one hundred and three of 1592 offspring carried exogenous DNA. The transgenic efficiency was 9.86% in 1995. With analysis by radioimmunoassay (RIA) determined the expression of human proinsulin in the milk of two transgenic sheep were 199.97mg/L and 183.62mg/L, respectively. Fluosence in situ PCR showed that the sperm of bull, goat and sheep can carry exogenous DNA after patient treatment method and exogenous DNA enter sperm nucleus and locate in the internal and peripheral nucleus of sperm. The average transfer efficiency of PRINS for sperm was 27.12% in sheep, 31.16% in bull, 30.57% in goat, respectably. We also found that the sperm must be washed completely to dispose the sperm fluid when using the patient method, because the DNaseⅠ contained in the fluid can digest the exogenous DNA." @default.
- W2390752816 created "2016-06-24" @default.
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- W2390752816 date "2001-01-01" @default.
- W2390752816 modified "2023-09-24" @default.
- W2390752816 title "Study for Production of Transgenic Sheep by Sperm Mediated Exogenous DNA Method" @default.
- W2390752816 hasPublicationYear "2001" @default.
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