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- W2390765781 abstract "Objective To explore the influence of hypoxia on the expression of nuclear factor Sp1 in the cultured human retinal pigment epithelial(RPE) cells in vitro.Methods To set up the hypoxic model,human RPE cells in vitro were cultured in the medium containing 200 μmol·L-1 CoCl2.At 0 hour(under normoxic condition),2 hours,4 hours,8 hours,12 hours and 24 hours after hypoxia,the location and dynamic changes of nuclear factor Sp1 in RPE cells were observed with cell immunofluorescence method and Western blotting,respectively,and the dynamic expression of Sp1 mRNA was detected by RT-PCR.Results The fluorescence of Sp1 protein mainly distributed in the cytoplasm of cultured RPE cells under normoxic conditions.With the time prolonged under hypoxic condition,green fluorescence intensity decreased within the cytoplasm,while the fluorescence intensity in the nucleus increased and to the highest level at 12 hours after hypoxia.The expression of Sp1 mRNA and protein were enhanced gradually with the time prolonged under hypoxic conditions,peaked at 12 hours and decreased at 24 hours in the cultured RPE cells but still higher than that under normoxic condition.Conclusion Hypoxia at early stage can increase Sp1 expression in cultured human RPE cells,and promote Sp1 protein transferring from the cytoplasm to the nucleus,which implies that Sp1 may play a role in the developing of hypoxic-ischemic chorioretinal diseases." @default.
- W2390765781 created "2016-06-24" @default.
- W2390765781 creator A5045035938 @default.
- W2390765781 date "2009-01-01" @default.
- W2390765781 modified "2023-09-28" @default.
- W2390765781 title "Expression of nuclear factor Sp1 in cultured human retinal pigment epithelial cells in vitro under hypoxic conditions" @default.
- W2390765781 hasPublicationYear "2009" @default.
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